The Heartworm Antigen Test is a serologic test that detects circulating female adult worm antigen. The test detects infected dogs as early as 6.5 months post-infection, but is most reliable at 8 months post infection. The antigen test is preferable to the Knott’s technique as it is more sensitive. In rare instances, the antigen test can give a false negative result in infected dogs. It is recommended to do a combination of Antigen Test and Knott’s Technique as this offers the greatest diagnostic power to detect infection. Routine testing of dogs should begin in the month of April each year.

Test Code:  HT-AG

Test Days:Tuesday and Friday
Turn Around Time (Days):1-5 days

1 ml serum or plasma


Serum or EDTA blood tube

Handling/ Storage:


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